Endocrine Abstracts

The regulation of TNF-α and IL-6 secretion in LPS-stimulated Peritubular cells was investigated in vitro. Immature rat Peritubular cells were incubated with LPS (10 μg/ml) over 24 h culture. TNF-α and IL-6 were measured in the culture medium by ELISA at 24 h, TNF-α and IL-6 genes by real-time RT-PCR, and activation of NF-κB, MAPKs, and AP-1 signal transduction pathways by western blotting. LPS stimulated the mRNA expression of IL-6 and much more the ex...

Atrazine is a chloro-s-triazine herbicide that has been in used worldwide for over 4 decades now. Its endocrine disrupting effects have been shown in mammals but the specific mechanism or mechanisms of action remain unknown. The aim of this study was to evaluate the acute effects of atrazine on testicular antioxidant systems and on some spermatological parameters in rats.Atrazine was administered to wistar rats at a dose equivalent to 120 mg/kg or 200 mg...